Effect of alcohol extract of watercress (Eruca Sativa) on human sperm motility duringin vitro sperm activation

Background: Herbal plants often contain active pharmacological compounds they are commonly used for the treatment and prevention of different diseases. Objectives The present study was aimed to identify the effects of different concentrationsof Erucasativa extraction (ESE) on human sperm motility for infertile male in vitro. Methods: Thirty five semen samples were obtained from men (mean age 27, range from 20-35 years) and prepared using centrifugation swim-up technique. Pro-SMARTmedium was used only in group1 (G1) as controls and two concentrations of ESE (50μg/ml and 100 μg/ml) were add to the culture medium of groups 2(G2) and3(G3) for in vitro sperm activation Results: Present study revealed that the sperm motility (%)was significantlyincreased(P˂0.05)post activation as compared to pre activation. Generally, group G3 showed best percentage of progressive sperm motility than G1 and G2. Conclusion The high concentration of ESE (100μg/1mL) improved humansperm progressive motility during sperm activation in vitro.